mutation
Keightley PD, Trivedi U, Thomson M, Kumar S & Blaxter ML 2009 Analysis of the genome sequences of three Drosophila melanogaster spontaneous mutation accumulation lines. Genome Res 19:1195-1201.
- we mapped a total of 174 single-nucleotide mutations, giving a single nucleotide mutation rate of 3.5 × 10−9 per site per generation
- the base composition of the genome is therefore not at an equilibrium determined solely by mutation
- the predicted G + C content at mutational equilibrium (33%) is similar to that observed in transposable element remnants
- nearest-neighbor mutational context dependencies are nonsignificant, suggesting that this is a weak phenomenon in Drosophila
- we also saw nonsignificant differences in the mutation rate between transcribed and untranscribed regions, implying that any transcription-coupled repair process is weak
- of seven short indel mutations confirmed, six were deletions, consistent with the deletion bias that is thought to exist in Drosophila
