genome assembly

Young AL, Abaan HO, Zerbino D, Mullikin JC, Birney E & Margulies EH 2010 A new strategy for genome assembly using short sequence reads and reduced representation libraries. Genome Res 20:249-256.

• the WGS assembly was conducted at the upper limit of current computational capabilities, requiring 256 G of memory compared with 32–64 G required for the RR approach

• where little is known about the genome content, gel electrophoresis analysis of genomic digestions using a variety of enzymes will likely yield robust results